First In vitro micropropagation of Vitis vinifera cultivars for production of virus free germplasm by apical meristem tip culture in Pakistan

The study aimed to develop a procedure for virus-free grapevine plant production through in vitro micropropagation. Collected explants from three grapevine cultivars were sterilized and grown on Murashige and Skoog medium with varying concentrations of cytokinins (BAP) and auxins (NAA and IBA).Different combinations of BAP and NAA were tested for shoot induction, while IBA and NAA combinations were used for root induction. This study demonstrates the successful production of virus-free grapevine cultivars using apical meristem tip culture, enabling the preservation of clean planting material and large-scale production of plantlets regardless of season.