Effect of high glucose on colorectal cancer cell proliferation by upregulating HGF/ C-Met gene expression

Objective : To investigate the effect of high glucose on colorectal cancer cell proliferation by up-regulating HGF/ C-Met gene expression. Methods : Colorectal cancer cells SW480 were cultured, proliferation activity was detected by CCK-8 method, invasion and migration of cells were observed by Transwell method, cell cycle distribution and apoptosis rate were detected by flow cytometry, and mRNA relative expression levels of HGF and C-Met in colorectal cancer cells were detected by RT-PCR. Results : Compared with blank group and control group, the proliferation ability of cells was enhanced by different glucose concentration for 24h, but the proliferation of cells was slow after 24h. By linear correlation analysis, the proliferation of HGF cells was linearly correlated with the expression of C-Met (r = 0.882, P < 0.05) at 24h after high glucose induction (R = 0.948, P < 0.01). There were statistically significant differences in the number of cell migration and invasion between blank group, control group, high glucose group and mannitol group (all P < 0.05). There were statistically significant differences in the proportion of cells in G0/G1 and S phases (P < 0.05), while there were no statistically significant differences in the proportion of cells in M phase (P > 0.05). The apoptosis rate of blank group, control group, high glucose group and mannitol group was statistically significant (P < 0.05). Compared with the blank group and control group, the expression level of HGF mRNA in high glucose environment increased significantly at the early stage of high glucose stimulation (6h), and continued to peak at 12h, then decreased and reached the lowest point at 48h, with statistical significance (P < 0.05). The expression of C-Met mRNA induced by high glucose was significantly different from that of the blank group and the control group (P < 0.05), and reached the peak at 24h after stimulation, then decreased gradually, and returned to the lowest point after 96h. Conclusion : High glucose can affect the proliferation, invasion and migration of colorectal cells which may be realized through up-regulation of HGF/ C-MET gene.