Antibiofilm Activity of Lactic Acid Bacteria of Probiotic Candidates Against Pathogenic Bacteria Streptococcus mutans and Enterococcus faecalis

Background Streptococcus mutans and Enterococcus faecalis are Gram-positive bacteria that play a role in the development of dental caries. These bacteria can form biofilm structures that inhibit the immune system and cause Multi-Drug Resistance. Lactic acid bacteria (LAB) produce organic acid compounds with potential probiotic. Methods In this study, Cell-Free Culture Supernatant (CFCS) LAB were derived from stingless honey bees with the following codes: Lacticaseibacillus rhamnosus strain TB-3 (PQ386429), L. rhamnosus strain HI-1 (PQ386478), Pediococcus acidilactici strain HI-5 (PQ386480), and P. acidilactici strain LT-3 (PQ386496). LAB compounds were tested for antibiofilm and antibacterial activity based on the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and in silico studies. Results CFCS TB-3 and LT-3 showed MIC values of 50% (v/v) against S. Mutans and 60% (v/v) against E. faecalis . TB-3 and LT-3 had MBC values ​​at higher concentrations than the other strains and had the highest antibiofilm activity at a concentration of 60% (v/v) against S. Mutans and 50% (v/v) against E. faecalis . In contrast, LAB metabolite compounds have a good affinity for the target protein that causes biofilms. Conclusion These results indicate that TB-3 and LT-3 are effective antibacterial and antibiofilm agents.