A nanoscale intertwined architecture within chloroplast of live Chlorella
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The precise spatial relationship between chlorophylls and flavoproteins is critical for photosynthesis but remains obscured by the diffraction limit. Using our developed laser scanning structured illumination microscopy on live Chlorella sp., we resolved their organization into a previously unseen intertwined nanoscale architecture, distinct from simple co-localization. We further show that this architecture is highly dynamic, undergoing significant remodeling under combined salinity and dark stress. These findings suggest the intertwined topology is a conserved, functional organizational principle for coordinating metabolism and photoprotection in vivo .
