Proteomic profile of Laser-dissected Motoneurons and Ependymal Cell Layer and of Dorsal Root Ganglia after Spinal Cord Injury in Rat
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Spinal cord injury induces profound molecular changes in surrounding tissue. Deciphering these changes with cell type-specific resolution shall facilitate discovery of new molecular targets that promote recovery. Here, we performed a proteomic analysis of laser-dissected motor neurons (MN) and ependymal cells (EC), dorsal root ganglia (DRG) obtained from adult control and spinal rats, 2 or 6 weeks after spinal cord transection at Th9. We traced with fluorescent cholera toxin and microdissected on average 172+/-39 MNs innervating soleus (SOL) muscle and 262+/-74 MNs innervating tibialis anterior (TA) muscle per animal. In parallel, we microdissected the EC layer that surrounds the central canal and the L3-6 spinal cord (the same levels as isolated MNs). We isolated the DRG bilaterally from L3, L4, L5 segments. Mass spectrometry analysis of the samples from 5 animals per group, allowed us to detect 1221 proteins in SOL MNs, 1186 in TA MNs, 1520 in EC layer and 5087, 3740 and 3086 in DRG L3, L4 and L5, respectively. Here we describe how this data was obtained and made available for further use. Our data may help to identify and characterize molecular mechanisms involved in early and late subacute period after spinalization in the rat spinal MNs, DRG and ECs. Design Type(s) parallel group design • injury design • disease process modeling objective Measurement Type protein expression profiling Technology Type laser capture microdissection • mass spectrometry assay • computer analysis with MaxQuant software Factor Type Procedure Sample Characteristic (s) Rattus norvegicus • lumbar motoneurons • DRG • ependymal cells of central canal
