Chelating Agents’ Effects on Dentin Properties and Bond Strength of Biodentine

This in vitro study evaluated the effects of different chelating agents on dentin properties influencing the adhesion of calcium silicate–based biomaterials. The effects of 9% etidronic acid (HEBP) and 0.2% chitosan nanoparticles on the microhardness, surface roughness, and push-out bond strength of Biodentine were assessed. Seventy-five extracted maxillary incisors were divided into three groups according to the final irrigation protocol: 9% HEBP, 0.2% chitosan, or 0.9% saline (control). Dentin microhardness (Vickers hardness number) and surface roughness (Ra, µm) were measured before and after irrigation. For push-out testing, standardized cavities in 60 dentin discs were filled with Biodentine and stored for 24 h at 37°C and 100% humidity. Failure modes were examined under a stereomicroscope. Data were analyzed using one-way ANOVA, Tukey HSD, Kruskal–Wallis, and paired t-tests (p < 0.05). All groups showed significant microhardness reduction (p < 0.001), greatest in HEBP. Surface roughness increased significantly, highest in HEBP (p < 0.001). Push-out bond strength was higher in HEBP than control (p < 0.01), with no difference between HEBP and chitosan (p > 0.05). Both agents improved Biodentine adhesion; chitosan produced milder dentin alterations, indicating potential for dentin preservation.