Screening of the Xylanase-producing Trichoderma Strain and the Optimization of its Enzyme Production Conditions

Purpose Xylanases have attracted considerable attention due to their excellent potential industrial uses. Results In this study, a xylanase producing strain was isolated from soil and identified as Trichoderma semiorbis Tsejk8, and the conditions for xylanase production were optimized. Additionally, two xylanase-related genes were cloned, and their functions were analyzed. The results indicated that the optimal conditions for xylanase production included maltose as the carbon source, peptone as the nitrogen source, an optimal pH of 6.0, and an incubation time of 120 h, yielding an enzyme activity of 40.7 U/mL. Following the purification of the protein via ammonium sulfate precipitation and ion exchange chromatography, four distinct protein bands were observed. Mass spectrometry analysis of these bands identified 14 associated proteins. Bioinformatics analysis revealed that two of these proteins belongs to GH3 (Glycoside Hydrolase family 3) beta- xylosidase. Conclusions In summary, the newly isolated strain Tasjk8 exhibits xylanase activity, which offers an effective and eco-friendly means of converting biomass into raw materials for industrial applications.