Radiosensitizing Effect of Gold Nanoparticles on MDA-MB-231 and MCF7 Breast Cancer Cells

Gold nanoparticles have been increasingly investigated as potential radiosensitizing agents for the treatment of breast cancer. In the present study, gold nanoparticles stabilized with sodium citrate and thioglycolic acid (AuNP-Cit-TGA) were synthesized to assess their radiosensitizing effects on MDA-MB-231 and MCF7 breast cancer cells after X-ray exposure. The AuNP-Cit-TGA nanoparticles displayed an average diameter of 22 nm, showcasing extremely low hemolytic activity in vitro, along with a moderate rate of cellular uptake by breast cancer cells. The uptake of AuNP-Cit-TGA notably decreases cell viability in both cell lines 48 hours after exposure to a radiation dose of 6 Gy, as demonstrated by the Alamar Blue assay. This decrease was associated with heightened oxidative stress, as evidenced by increased DCFH fluorescence measured 24 hours following a 2 Gy exposure. Remarkably, the uptake of AuNP-Cit-TGA enhances the occurrence of single-strand DNA breaks in MCF7 cells by nearly double when compared to radiation treatment alone; however, this effect was not statistically significant in MDA-MB-231 cells. Despite this, residual γH2AX and 53BP1 foci caused by ionizing radiation were observed in both cell lines, revealing ongoing unrepaired DNA double-strand breaks and genomic instability connected to the uptake of nanoparticles by cells. Collectively, these findings provide valuable insights into the application of AuNP-Cit-TGA as an effective radiosensitizing agent in breast cancer radiotherapy.