Drynachromoside A from Drynaria roosii induces proliferation and differentiation of MC3T3-E1 osteoblasts involving Wnt/β-catenin signaling pathway

Alveolar bone loss belongs to jaw bone defect and is the determinant for the overall restorative outcome and long-term stability of various denture restorations including implant-supported dentures. After tooth extraction or periodontitis, alveolar bone loss often appears. In clinical practice, there are no ideal therapeutic drugs for alveolar bone formation since drugs for osteogenesis usually present side effects. Therefore, discovery of novel therapy is essential. Drynaria roosii Nakaike is a medicinal plant used for osteogenesis in Chinese folk. To search novel phytochemicals targeting alveolar bone loss, we have explored phytochemicals in D. roosii and assessed the effects using MC3T3-E1 cells, which results in the identification of drynachromoside A. Further investigations showed drynachromoside A induced proliferation, osteogenesis and adhesion of MC3T3-E1 cells. and these effects were associated with activation of Wnt/β-catenin signaling pathway. These results could provide evidences for the discovery of novel therapy targeting alveolar bone loss and application of D. roosii in practice.