Chemical constituents Antimicrobial activity Cytotoxicity of Moringa oleifera Seed Extract Grown in AL Mukhwa at Al-Baha Area, Saudi Arabia

Background - Almukhwa is located in Al-Baha area (Tohama area) in Saudi Arabia that is rich in natural products such as Moringa oleifera, which now have high demand in the world. Seed extract of Moringa oleifera exhibits chemo-preventive properties used to inhibit the growth of pathogenic bacteria (gram positive, gram negative and fungi) and human cancer cells. Methodology - The biochemical characterization was carried out according to the gas chromatography (GC) and HPLC. The antibacterial activity tested by Agar well diffusion, for antibacterial activity using nutrient agar and for antifungal activity using potato dextrose agar, for the determination of the minimum inhibitory concentration and minimum bacterial concentration (MIC), (MBC) the method of dilution in liquid medium was used. The MTT protocol was used to determine the cytotoxicity of moringa seed on cancer cells. Results - The bioactive constituents of moringa seed extract examined by Gas Chromatography-Mass Spectrometry (GC-MS) were found 10 compound 4 of them were major compound tridecanal (3.76), n-Hexadecanoic acid (40.01), 9-Octadecenoic acid (Z)-, methyl ester (4.46) and Oleic Acid (44.69), also the Polyphenol constituents examined by HPLC contained gallic acid, Chlorogenic acid, Methyl gallate, Coffeic acid, Syringic acid, Rutin, Ellagic acid, Coumaric acid, Vanillin, Kaempferol which have high microbial activity to different pathogenic organisms, gram positive Enterococcus faecalis (ATCC 29212 ), Staph aureus (ATCC 6538) , gram negative Salmonella typhi (ATCC 6539 ), Escherichia coli (ATCC 8739), fungus Candida albicans (ATCC 10221) compare with antibiotic Gentamicin for bacteria and Fluconazole for fungi except Pseudomonas aeruginosa (ATCC 90274) and Aspergillus niger (AUMC 14260) have low activity, also have cytotoxicity against cancer cell line such as colon cancer (HCT116), pancreatic duct carcinoma (PANC1) and Kasumi1 a myeloblast cell isolated from the peripheral blood of an Acute myelomyeloplast leukemia at highest concentration of moringa seed extract (1000 Ug/ml).