Histone H3 post-translational modifications in Aedes aegypti during Dengue virus infection
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This study provides the first comprehensive profile of histone H3 post-translational modifications (PTMs) in midguts of Aedes aegypti females infected with DENV-2. A reference strain with susceptibility to infection was selected, as confirmed by the detection of viral load in midguts and excreta. Nuclear histones were subsequently extracted, and 21 H3 modifications were evaluated using a multiplex ELISA assay. Infection induced marked increases in activating methylations such as H3K4me2 (+77.6%) and H3K4me3 (+34.9%), as well as in H3K9me1, H3K9me2, and H3K36me2. Moderate increases were observed in other methylations, acetylations (H3K9ac, H3K14ac, H3K18ac, H3K56ac), and phosphorylations (H3Ser10P, H3Ser28P), whereas H3K4me1 showed a sustained decrease. Other marks displayed variable patterns across replicates. These findings provide a first insight into DENV-2–induced epigenetic remodeling in Aedes aegypti, offering novel insights into the dynamics of H3 histone modifications in a model with limited prior knowledge.
