Biodiversity Analysis of the Human Gut Microbiome in Healthy Individuals Using Bioinformatics Tools

Motivation : The human gut microbiome plays a crucial role in maintaining health and preventing disease. This study aims to provide a comprehensive analysis of the gut microbiome composition in healthy individuals using integrated bioinformatics tools.We analyzed gut microbiota samples from ten healthy individuals using UGENE software, employing taxonomy tools such as Kraken, DIAMOND, and MetaPhlan. Alpha diversity indices, including the Shannon and Simpson diversity indices, were calculated. Correlation analyses were performed to explore relationships between microbial diversity, age, and geographic living conditions. Results : The gut microbiome showed significant diversity across samples. Alpha diversity indices indicated high microbial diversity, which tended to decrease with age. Individuals living in mountainous regions exhibited lower diversity than those in villages.This study highlights the complex diversity of the human gut microbiome and its variation with age and geographic location. The presence of both indigenous microbiota and pathobionts genera can lead to possible dysbiosis within the gut ecosystem. High microbial diversity is associated with better health outcomes, emphasizing its importance in maintaining gut health. Future research should aim to further elucidate the functional roles of these microbial communities.