Evaluation of Antioxidant and Spectroscopic analysis of Psoralea corylifolia and Nigella sativa Plant Extract

Background Analytical methods of UV spectroscopy, FTIR, TLC and antioxidant activity are used to test the drug and active constituent in the laboratory to determine how much active constituent is present in the test drug through these methods. Aim In UV estimation, the test at 254 nm has a very sharp peak, which represents that the test drug is pure, which shows that this nm is present in the psoralene active constituent that is present in the psoralene corylifolia and In FTIR analysis of the sample, how many functional groups are present in the test sample. Methods In TLC analysis, a different mobile phase was prepared, and all the samples were run, but in one phase, the test samples ran excellently, and separation was good (hexane: ethyl acetate) in a ratio of 8.5:1.5 v/v. Compared to the test drug with an active constituent, the RF valve is the same. Result In antioxidant activity, antioxidant properties with DPPH and compare them to the standard antioxidant drug, Gallic acid. In this activity we prepare the different concentrations of the solution and check which concentration shows the high antioxidant activity and compare it with the standard drug concentration activity. Conclusion The test drug antioxidant property is high in the 100 µL concentration. The sample is run in UV-visible spectroscopy at 517 nm, and the standard is run at 270nm for the absorbance of the drug. Major Finding: The study revealed that both Psoralea corylifolia and Nigella sativa extracts exhibit strong antioxidant activity, with Nigella sativa showing slightly higher free radical scavenging potential. Spectroscopic analysis confirmed the presence of phenolic and flavonoid compounds contributing to their antioxidant properties.