Prevalence of dog's Echinococcus granulosus with study of the Genetic Variability of Antigen B2

Echinococcosis is caused by infection with tapeworms, represented by Echinococcus granulosus. The antigen B is a main protein produced by the metacestode of this parasite and is a member of a family of genes that help evade the host's immune system. There are many different types of E. granulosus (G1-G10), each with its own unique set of biological traits.in this study we investigate of sequencing and polymorphism of AGb2 of Echinococcosis in final host of dog. Two hundred and seventy dog ​​feces samples were examined microscopically to detect the parasite eggs, and then DNA extraction was done for each positive sample, where conventional PCR technique used specific AgB2 gene primer and gene sequencing to detect E. granulosus strains. The results of the microscopic examination indicated that 24 (8.8%) of the cases were positive (13 males and 11 females), including 8 puppies and 16 adults. That 17 out of 24 succeeded in amplifying the DNA and two sequences were submitted and gotten accession number OP272485.1, OP272486.1 and aligned with group of AGB2 groups. The results showed AgB2 play functional genes exclusively in dog as final host of E. granulosus , suggesting inter-strain variation of this gene.