Spectroscopic analysis of the interaction between human serum albumin and newly synthesized quinoline derivatives
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The studies on synthetic derivatives of known compounds are an important part of the search for new substances with potential therapeutic activity. The aim of this study was to investigate the spectroscopic properties as well as antioxidant activity of newly synthesized quinoline derivatives QDs (QuiC 3 H 7 , QuiC 7 H 7 , QuiC 6 H 5 , QuiC 8 H 9 ) and their influence on the HSA`s conformation. To achieve it, spectroscopic techniques (UV-Vis spectrophotometry and circular dichroism spectroscopy) were used. All compounds showed the ability to absorb UV-Vis radiation and the presence of both transitions π-π* and n-π*. The antioxidant activity of QDs can be described as follows: QuiC 7 H 7 = QuiC 8 H 9 < QuiC 3 H 7 < QuiC 6 H 5 and the observed divergence in the antioxidant activity of the QDs was most likely due to their structural differences. None of the included ligands statistically significantly changed the percentage of α-helix in the HSA molecule while almost QDs caused statistically significantly changes the near-UV CD spectrum, especially in Region 1st, Region 2nd, Region 3rd. QuiC 3 H 7 and QuiC 7 H 7 induced conformational changes in the environment of all aromatic amino acid residues, including the Trp-214 residue. In contrast, QuiC 8 H 9 did not induce changes in the HSA`s spectrum (Region 3rd ).Newly synthesized quinoline derivatives (QDs) have never been synthesized or studied before, therefore the obtained results constitute a novel achievement and it is likely that the tested QDs will find application as therapeutic substances in the future.
