Unveiling novel longevity-regulating mechanisms of phytogenic adaptogen Phytocee TM through a computational biology approach

Phytogenics is a promising and novel sector in animal health. Indian botanicals have been shown to protect animals from various diseases and health stressors, which include nutritional, environmental, biological, and chemical stressors. These stressors may cause sudden mortality in the animals and are a matter of concern among farmers, which Phytocee ^TM can address. It consists of scientifically validated (LC-MS/MS) phytochemicals belonging to the classes of triterpenoids, hydrolysable tannins, and withanolides that help enhance the adaptogenic potential of the animals. The adaptogenic potential of an animal is associated with prolonging their lifespan. This research investigated the pathway-focused approaches for increasing longevity in animals at a cellular level by observing their differentially expressed genes (DEGs): FOXO, AMPK, SIRT1, and mTOR using computational tools for gene enrichment: DIGEP Pred2.0, which showed significant modulation of the DEGs (1.25e-159), especially for the enriched term “longevity regulating pathway”. Pathway mapping using SHINYGO0.82, KEGG, showed significant mapping of these genes in the enriched pathway. Network pharmacology was done to detect protein-protein interactions for these DEGs using INPUT2.0. The heat maps and flowerpot Venn diagrams were generated for enriched pathways using SR-PLOT, which showed that the maximum genes were responsible for regulating the longevity of the animals. The study concluded with a possible molecular pharmacology of Phytocee ^TM, suggesting that the product may have helped overcome stressors by increasing the lifespan of various animal species, including poultry, swine, and aquatic animals. The possibility can be further validated through the wet lab trials.