Effects of PEG-6000 Simulated Drought Stress on Germination of Five Derivative Lines of wheat-Leymus mollis
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To explore the drought tolerance of five wheat materials (H9, H24, H29, H30 and H47) and provide a basis for breeding drought-tolerant wheat. 5%, 7.5% and 10% Polyethylene glycol (PEG-6000) solutions were used to simulate drought environments.The results indicated that under drought stress, growth indices-including germination energy (GE), germination rate (GR), germination index (GI), germination drought resistance index (GDRI), root length (RL) and seedling length (SL)-exhibited a downward trend. Chlorophyll (Chl) content decreased with increasing stress concentration, among the materials, H47 showed the smallest decrease compared with the corresponding control group under different drought stresses (3.07%, 14.40% and 25.82% respectively). Malondialdehyde (MDA) and proline (Pro) contents generally increased with an higher PEG-6000 concentrations. Using the membership function method for comprehensive evaluation, the drought tolerance ranking of the five wheat materials was determined as H47 > H9 > H24 > H29 > H30, with H47 showing the highest comprehensive drought resistance score and the strongest drought tolerance. In conclusion, this study can provide excellent germplasm resources and basic research materials for breeding drought-resistant wheat varieties and mining drought-resistant genes.
