Caspia-LAMP - species-specific LAMP-based tool for the molecular detection of Cordylophora caspia (Pallas 1771) (Cnidaria, Hydrozoa)

The rapid detection of invasive species is crucial for effective management and mitigation of their ecological and economic impacts. Cordylophora caspia is an invasive hydroid that has successfully colonized freshwater and brackish ecosystems worldwide, causing significant environmental and infrastructural problems. Traditional morphological identification methods are often insufficient for early detection, particularly when populations are sparse or specimens are damaged or inappropriately preserved. Here, we present the validation of a Loop-Mediated Isothermal Amplification (LAMP) protocol specifically designed for the detection of C. caspia DNA (Caspia-LAMP). The method targets unique repetitive DNA sequences identified through genome analysis, enabling highly specific detection without cross-reactivity with other cnidarians or co-occurring invasive species. Our optimized protocol demonstrates remarkable sensitivity, capable of detecting as little as 10 ^− 7 ng of C. caspia DNA, surpassing conventional PCR methods. This rapid, sensitive, and field-applicable method represents a valuable tool for early detection and monitoring of C. caspia populations, potentially enabling more timely and cost-effective management interventions. The successful development of this protocol also serves as a model for creating similar molecular tools for other invasive species detection and monitoring programs. Clinical trial number: not applicable.