Lipolytic potential of gut isolates in silkworm Bombyx mori. L

The current study describes that the bacteria isolated from the midgut of the silkworm Bombyx mori had the ability to degrade the oil form of lipids and could produce the lipolytic enzymes that hydrolyse the lipids into simpler compound form that could be uptake by the bacteria as their energy source (carbon source), which had been evidenced primarily by the Tributyrin and TWEEN 20 Agar plate assay method. Further, the six isolates were selected from the primary screening process and so the growth curve assays of Olive oil and Tributyrin Medium were performed, which showed that the isolates utilised the lipid substrate; therefore, the cell densities were increased gradually from 12 hours, 24 hours and 48 hours of Tributyrin Medium and quiet difference in the Olive oil Medium. The FTIR analysis and the HPLC analysis reported that the six isolates utilized and lysed the lipid substrate in the tributyrin and olive oil medium, which was confirmed by the peaks obtained from the six isolates compared with their respective controls. From all the reports, isolate Serratia spp was very active compared with the other five isolates, so the effect of the pH of isolate Serratia spp was tested on the phosphate buffer and tris buffer where the isolate Serratia spp were very active on the phosphate buffer. Among the six isolates, the most potent lipolytic isolate was Serratia spp .