Sample Preparation for Green Isolation of Bioactive Furanoflavonoid and Disaccharide from Pongamia pinnata seeds

Pongamia pinnata popularly known as Karanj, is a legume. The traditional and pharmacological properties of Karanj are well known. Karanjin a bioactive furanoflavonoid is the major constituent of karanj seeds. The present research work aimed at sample preparation for green isolation of furanoflavonoid (karanjin) and disaccharide (sucrose) with an emphasis on achieving a higher yield of extracts, fractions and pure compound using different methods. The samples for isolation of compounds were prepared by the process of fractionation. The methanol extract of P. pinnata seeds was fractionated with four different solvents (hexane, chloroform, butanol and water).The chloroform fraction and methanol extract were used for green isolation of karanjin and sucrose by crystallization. The identification of karanjin was done by melting point, HPLC analysis and NMR spectroscopy and sucrose by melting point and NMR as reported in literature. The antimicrobial activity of karanjin against Mycobacterium kansasii was reported for the first time with MIC of 0.05 µg/ml. Thus a successful attempt for isolation of compounds without use of column chromatography and large amount of solvents was made in this research work. The isolation of sucrose from water fraction in large amount has economic value whereas isolation of karanjin in higher yield from chloroform fraction has medicinal value.