β-Cyclodextrin Stationary Phase Using Tetrafluoroterephthalonitrile as Spacer Arm for Separation of Phenolic Compounds

In the field of chromatographic separation, high-performance liquid chromatography (HPLC) is widely used in the analysis of complex samples due to its high separation efficiency, fast analysis speed and wide application range. The core goal of the approach was to develop a more efficient chromatographic stationary phase. Although cyclodextrin chromatographic stationary phase has been reported previously, there are various challenges that prevent it from becoming the mainstream chromatographic stationary phase, such as low bonding amount, complicated synthesis process and poor separation effect. In this paper, a novel β-cyclodextrin chromatographic stationary phase was prepared with polysubstituted tetrafluoroterephthalonitrile (TFTPN) as a spacer arm for the separation of phenolic compounds. The chromatographic results showed that it had excellent chromatographic performance, and the mechanism of action was explained by computer simulation. This study provides new insights for the separation and analysis of structural analogues.