Coffea arabica Pulp as a Natural Antioxidant to Improve Cryopreserved Brown Swiss Bull Semen Quality: A Pilot Study

  1. Edson O. Castro Rios
  2. Marilu Mestanza Mendoza
  3. Deiner Jhonel Gongora Bardales
  4. Jonathan Alberto Campos Trigoso
  5. Hugo Frias Torres
  6. Segundo José Zamora Huamán
  7. Rainer Marco López Lapa
  8. Lizeth Amparo Heredia Vilchez
  9. Nilton Luis Murga Valderrama
  10. Gleni Tatiana Segura Portocarrero
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Abstract

Cryopreservation induces oxidative stress in bovine semen, compromising sperm quality. This pilot study evaluated the antioxidant potential of Coffea arabica pulp (CAP), an agro-industrial by-product, on refrigerated and cryopreserved Brown Swiss bull semen. Ten ejaculates were diluted in Optixcell® and supplemented with CAP (0, 1, or 2 mg/ml). Sperm motility and kinematics (CASA), membrane integrity (HOST), and acrosomal integrity (Coomassie blue) were assessed. CAP at 1 mg/ml (T1) significantly improved progressive motility (81.2%) in cryopreserved samples compared with the control (62.6%, p < 0.05). Kinematic parameters (VAP, VSL) were also higher in T1 than in the control (p < 0.05). Acrosomal integrity was improved in T2 (88.8%, p < 0.05), while membrane functionality increased in refrigerated semen with T1 (p < 0.05). These findings indicate that CAP may act as a natural antioxidant to enhance semen cryopreservation outcomes. Further studies with larger sample sizes and fertility trials are warranted.

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  1. Version published to 10.21203/rs.3.rs-7457706/v1 on Research Square