Exploring the Mechanism of Regulating Tubal Inflammatory Infertility through TGF- B1/Smads/CTGF Signal Transduction Pathway by Promoting Blood Circulation, Removing Stasis, and Tonifying collaterals Method Based on Bioinformatics and Molecular Docking

Purpose: Based on bioinformatics and molecular docking, this study explores the mechanism of promoting blood circulation, removing blood stasis, and unblocking collaterals through the TGF-B1/Smads/CTGF signaling pathway to regulate tubal inflammatory infertility. Methods: Collect data from OMIM database, RefSeq Gene database, TTD database, CTD database, and Genecards database. Collect 12 medicinal ingredients and targets of modified prescriptions for promoting blood circulation, removing blood stasis, and unblocking collaterals using the TCMSP database. Collect common targets using Venn diagram and perform GO and KEGG analysis. Select relevant representative targets and corresponding components for molecular docking. Results： There are 37,176 target points for tubal inflammatory infertility disease. Through pharmacological analysis, there are 343 corresponding targets for 14 related traditional Chinese medicine herbs using the method of promoting blood circulation, removing blood stasis, and unblocking collaterals. There are 326 common targets with tubal inflammatory infertility disease. The top 20 key targets including AKT1, TNF, IL6, IL1B, TP53, EGFR, ESR1, PTGS2, STAT3, SRC, CASP3, BCL2, NFKB1, HIF1A, MMP9, CTNNB1, IFNG, ICAM1, STAT1, and NFKBIA were obtained through Cytoscape software analysis. It involves signaling pathways such as WP5434, hsa05417, hsa05208, WP5420, WP5470, WP2882, WP4298, hsa05207, WP4396, hsa05418, etc. Conclusion: The method of promoting blood circulation, removing blood stasis, and unblocking collaterals can regulate tubal inflammatory infertility through multiple targets and pathways, including the TGF-B1/Smads/CTGF signaling pathway.