Physico-chemical Properties of Silk Sericin Extracted by Hydrothermal Treatment

Sericin is a natural protein and is currently used as a biologically active substance in medicine, pharmaceuticals, the food industry, and tissue engineering. Therefore, in this research work, research was conducted on the extraction of sericin with a high degree of purity from natural silk by hydrothermal hydrolysis and the study of its physicochemical properties for the fields of biomedicine, pharmaceuticals, and cosmetology. The process of extracting sericin from natural silk by hydrothermal hydrolysis in water in the temperature range of 110 and 100°C was carried out for 2–24 hours, and sericin extracted molecular weight between 20 kDa and 240 kDa, product yield of 10-29.9%. We determinate amino acid content, free amino and carboxyl groups by analytical methods, also, its physicochemical properties were studied by modern research methods. There are possibilities for obtaining biomaterials used in medical practice for the treatment of inflammatory skin diseases, as well as biologically active additives aimed at the prevention and treatment of type II diabetes mellitus, by binding high-purity sericin with polysaccharides (dialdehyde carboxymethylcellulose) in aqueous conditions. The pure fibroin that was obtained can be used to produce polyfunctional hemosorbents that contain reactive functional groups in their fibrous composition. These hemosorbents are designed to purify blood and blood serum from harmful substances using the hemosorption method.