Investigation of Chikungunya Arbovirus in Cities of Bushehr Province: An ELISA-Based Study: A Cross- Sectional Study

Background: Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that poses a public health threat in regions suitable for Aedes mosquito proliferation. This study aimed to assess the seroprevalence of CHIKV in Bushehr Province, Iran. Methods: A cross-sectional study was conducted from July to September 2023 across ten cities in Bushehr Province. A total of 180 serum samples were collected from blood donors and hospital attendees. CHIKV-specific IgG antibodies were detected using a commercial ELISA kit, and results were interpreted per the manufacturer’s guidelines. Results: Out of 180 samples, 5 tested positive for CHIKV IgG antibodies, indicating a seroprevalence rate of 2.78%. Positive cases were identified in Bushehr, Dashtestan, and Dashti cities, suggesting limited but detectable exposure. Conclusion: Although CHIKV prevalence was low, the presence of seropositive cases indicates potential risk for virus introduction and local transmission. Given the favorable climate for Aedes mosquitoes in the region, continued vector surveillance and preventive public health measures are recommended.