CCT7 indicates a poor prognosis and is associated with immune infiltration in colonic adenocarcinoma
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Objective CCT7, a member of the t-complex polypeptide 1 chaperone family, facilitates protein folding in an ATP-dependent manner. To date, the role of CCT7 in the onset and progression of malignant tumors remains unclear. This study investigated the expression of CCT7 in colonic adenocarcinoma (COAD) and its role in the initiation and development of COAD. Methods The bioinformatic databases were used to examine the CCT7 expression in COAD, and the results were verified using human clinical specimens by immunohistochemistry (IHC) assay. The association of CCT7 expression with prognosis was evaluated by Kaplan-Meier method and Cox regression analysis. Gene Ontology-Biological Process (GO-BP) enrichment analysis was used to investigate the potential biological functions of CCT7 in COAD. CCK-8, colony formation and Transwell assays were carried out to explore the effect of CCT7 on proliferation, migration and invasion of human COAD cells. The correlations of CCT7 expression with immune infiltration, immunotherapy and drug sensitivity were determined by single sample gene set enrichment analysis (ssGSEA) and correlation analysis. Results CCT7 expression was up-regulated statistically in COAD tissues compared with normal colonic tissues ( P < 0.05) and high expression of CCT7 predicted poor prognosis of COAD patients ( P < 0.05). GO-BP enrichment analysis revealed that CCT7 was mainly involved in the processes of cell proliferation and microtubule activities( P < 0.05), and down-regulation of CCT7 inhibited the proliferation, migration and invasion of COAD cells ( P < 0.05). CCT7 was negatively associated with infiltration of most immunocytes and the scores for PD-1 and CTLA-4 therapy ( P < 0.05). Moreover, drug sensitivity analyses showed that CCT7 affected the sensitivity of COAD samples to several anti-cancer drugs ( P < 0.001). Conclusion CCT7 may function as an oncogene to promote the malignant phenotype of COAD, and serve as a promising biomarker for prognosis as well as a reference for clinical treatment in COAD.