A novel narnavirus isolated from the edible fungus Pholiota nameko
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A novel positive single-stranded RNA virus, tentatively named “Pholiota nameko narnavirus 1” (PnNV1), has been identified in an edible fungus Pholiota nameko strain N1. The complete genome of PnNV1 is 2254 nucleotides in length, containing a single large open reading frame (ORF) that encodes an RNA-dependent RNA polymerase (RdRp) of 682 amino acids (aa) with a molecular mass of 77.81 kDa. blastp analysis indicated that PnNV1 exhibited the highest similarity (30.80%) to Rhizoctonia solani narnavirus 18. Sequence alignments and phylogenetic analysis based on RdRp sequences indicate that PnNV1 can be classified as a new member of the family Narnaviridae . To our knowledge, this is the first mycovirus reported in the edible fungus Pholiota nameko .