In vitro Sun Protection Factor calculation using the Mansur method and Its uncertainty for Sintrong leave without extraction stage

Sun protection factor (SPF) value is essential information for UV protection. Natural resources have been a good potential SPF for UV protection. However, the testing method for the SPF value was less efficient if an extract sample was used. In this study, the method was evaluated the SPF value using the Mansur method and its uncertainty, and introduced the non- extract sample (Sintrong leave as model). After UV-visible spectrophotometer measurement, the result showed that the most SPF Value was 28.8733 ± 0.2382 (moderate category) from the solution of 1000 ppm. This developed method was acceptable based on Linearity, LOD, LOQ, and precision. The profiles of UV graph results showed the activity as sun protection at 290 – 320 nm with their characteristic peaks.