Antibiotic Sensitivity Pattern of Elective Surgeries for Postoperative Wound Infections in a Tertiary Care Centre

Background In this study, surgical site infections that isolate, identify, and investigate the pattern of antibiotic sensitivity in surgical wound infections that occurred after surgery in a tertiary care center. Methods During this research 104 swab samples were collected from different performed surgeries such as exploratory laparotomy, Laparoscopic cholecystectomy, and Colectomy, Hernia, Ulcer, and Cancer patients and then analyzed for culture isolation and identification using selective culture media. The patients' were between the ages of 10 to 90 years, with 54 samples collected from males and 50 from females and further biochemical characterization have done. For Staphylococcus aureus , the samples were cultured on Mannitol Salt agar (MSA) medium which showed golden-yellow zone colonies with mannitol fermentation on media and cetrimide agar for Pseudomonas aeruginosa that showed greenish-blue pigmented colonies on due to pyocyanin production on media. S. aureus showed positive test for catalase and coagulase, while P. aeruginosa showed positive results for oxidase and citrate utilization. To determine each isolate’s antibiotic sensitivity pattern, the standard Kirby-Bauer disc diffusion method were utilized in accordance with the clinical and laboratory standard institute (CLSI) guidelines. Result Out of 104 swab samples, 96 showed positive bacterial growth, while 8 exhibited no growth. Among the culture-positive samples, S . aureus was isolated in 18 cases (13.10%) and P . aeruginosa was isolated in 24 cases (17.40%). Further, 33.33% isolates of S. aureus and 37.50% isolates of the P. aeruginosa showed multidrug-resistant (MDR). However, antibiotic susceptibility testing revealed that S. aureus showed 100% sensitivity to linezolid and 72.22% sensitivity to tetracycline while P. aeruginosa showed 75% and 70.83% sensitive to ofloxacin andpiperacillin, respectively. Conclusion Based on these findings, linezolid and ofloxacin may be considered the most effective antibiotics for treating surgical site infections caused by S. aureus and P. aeruginosa , respectively.