Interaction of CP and NCP BVDV with bovine sperm cells on apoptosis and oxidative stress in vitro

Background BVDV is one of the most infected viral diseases which can impact on genital system of male and female cows. The goal of this study was to see how CP and NCP biotypes of BVDV affected on spermatozoa in vitro . Materials and methods BVDV-free frozen sperm cells were counted and centrifuged to separate viable sperms. CP and NCP BVDV with 3 different doses of 10 ⁵ , 10 ⁴ and 10 ³ TCID 50/mL were incubated to10 ⁵ sperm/ml for 2 hours. To assess oxidative stress and apoptosis, the luminescence technique was used to quantify ROS, Caspases 7/3 and 9, as well as ATP. They were repeated 3 times. Results The effect of virus biotypes on plasma membrane integrity has led to a significant drop in both 10 ⁵ and 10 ³ CP and NCP BVDV concentrations (P ≤ 0.05). The impact of 10 ⁵ dose of CP biotype compared to 10 ⁴ and 10 ³ doses of the same biotype was significant (P ≤ 0.05), but no significant difference was observed between the effect of 10 ⁴ and 10 ³ doses. Caspase 3/7 produced showed a significant effect (P ≤ 0.05) of both biotypes on sperm cells apoptosis. Caspase 9 produced showed a significant effect (P ≤ 0.05) of the three dosages of CP in addition to the high dose of NCP on apoptosis. ATP produced showed a significant effect (P ≤ 0.05) of 3 dosages. The effect of 10 ⁵ of CP compared to 10 ⁴ doses and 10 ⁴ to 10 ³ doses were significant (P ≤ 0.05). Conclusion We concluded that BVDV biotypes could affect the vital aspects, dynamic features and also induction of oxidative stress and apoptosis on bovine spermatozoa cells in vitro .