Assessing the extent of multisectoral collaboration in antimicrobial resistance interventions

Introduction Antimicrobial resistance (AMR) is a global health risk of the 21st century, compromising the efficacy of antibiotics and other antimicrobial medicines vital to human and animal well-being. Containment of AMR requires robust multisectoral collaboration (MSC). We determined the extent to which MSC in strengthening AMR interventions in Uganda occurs. Methods We conducted a cross-sectional study among AMR stakeholders in Uganda during November 2024–February 2025. We included adults aged ≥ 18 years using purposive sampling. Multisectoral collaboration was measured using the WHO’s Tripartite AMR Country Self-Assessment Survey (TrACSS) questionnaire that was self-administered to participants. Data on sociodemographic factors, and MSC for AMR were collected. Multisectoral collaboration was categorized as either being non-existent, existent or established. Results A total of 166 participants were included, with 49% being aged 31–40 years and most holding at least a master’s degree (53%). Multisectoral collaboration for AMR was established among 33% of the participants and exists among 55% of the stakeholders. While most participants (80%) reported relevant sector representation, and 85% noted the existence of a national strategic plan, only 44% cited shared leadership, and 40% reported active multisectoral preparedness. Intersectoral coordination remained limited, with 31% confirming structured collaboration, 22% reporting intra-sectoral collaboration, and 36% recognizing international partnerships. Gaps were observed in resource availability, data infrastructure, and internal communication, with 7% reporting a dedicated MSC budget, 1% citing data sharing agreements, and 39% acknowledging internal communication systems. Conclusions Although MSC for AMR interventions exists among the majority of stakeholders, its establishment remains limited, with gaps in leadership, preparedness, coordination, resources, data infrastructure, and internal communication. Strengthening governance structures, investing in dedicated resources, and enhancing coordination and data-sharing mechanisms are essential to fully operationalize and sustain effective MSC for AMR. Clinical trial number: Not applicable