Exploring Nutritional Evaluation of Cauliflower (Brassica Oleracea Var Botrytis) Waste on the Thyroid Hormone, Antioxidant Status, Immunity and Expression of Interleukin 6 and HSP 70 Genes in Broilers

The present study was investigated effect of CLM on thyroid hormone profile, immunity, expression of IL6 & HSP70 gene and antioxidant status of broiler. 240 day-old chicks were distributed into 5 groups having 8 birds in each replicate. The diet was formulated as per BIS (2007) standards for T1, while T2, T3, T4, T5 and T6 were given diets substituted with CLM @ 50, 75, 100, 125, and 150 g/kg, respectively for 42 days. The weight of the spleen, thymus and humoral immune response was significantly (P < 0.05) higher in T6 group. On 21st day, serum IL6 levels was significantly (P < 0.05) lower in T4, T5, and T6. On 42nd day, it was significantly (P < 0.05) lower in T6. Relative expression of the HSP70 gene in liver tissue was significantly (P < 0.05) down regulated in T6. The serum SOD and catalase levels were significantly (P < 0.05) higher in T4, T5, and T6. Serum FRAP and DPPH values were significantly (P < 0.05) higher in T5 and T6. The thyroid hormone profile, weight of bursa, cell-mediated immune response and serum GPx levels were remained comparable. Hence, it is concluded that CLM inclusion in a diet up to 15% improves immunity and antioxidant and alleviates heat stress in chickens.