Root colonisation and induction of plant defence-associated signalling pathways in Arabidopsis thaliana by Serratia marcescens, Streptomyces galilaeus, and Trichoderma viride
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Some microorganisms that are antagonistic to phytopathogens can activate induced systemic resistance (ISR) in plants. We sought to determine whether Streptomyces galilaeus CFFSUR-B12, Serratia marcescens CFFSUR-B2, and Trichoderma viride CFFSUR-A21 – strains recognised for their antagonistic capacity – could colonise the roots of and induce resistance in Arabidopsis thaliana . The antagonistic activity of Colletotrichum spp. was determined in dual-culture assays. Strains were inoculated separately in the roots of A. thaliana to study root colonisation and activation of ISR in leaves. PR1 and PDF1.2 expression was monitored by RT-qPCR in leaves. Serratia marcescens CFFSUR-B2 colonised the rhizoplane and endorhizosphere, whereas Str. galilaeus CFFSUR-B12 and T. viride CFFSUR-A21 only colonised the rhizoplane. Serratia marcescens and T. viride induced co-expression of PR1 and PDF1.2 , while Str. galilaeus induced only PDF1.2 expression. These findings reveal new avenues for research into plant disease management in the humid tropics.
