Metabolomic analysis o1f alizarin intervention on duck enteritis virus-infected duck fibroblast cells

(background）Alizarin is the main active component in traditional Chinese medicines such as Rubia cordifolia and Lonicera japonica, and it has anti-inflammatory and antiviral effects. It has been demonstrated that Rubia cordifolia can effectively prevent and treat duck enteritis virus (DEV) infection. This study aims to further clarify the mechanism by which rubiadin prevents DEV infection. (Method)Duck embryo fibroblast (DEF) cells were pretreated with alizarin before being infected with DEV. Cell samples were collected at different time points for metabolomics sequencing, and the proliferation of DEV was detected by qPCR simultaneously. (Result）Compared with the virus infection group, the alizarin intervention group exhibited a significantly reduced DEV load in DEF cells (P < 0.05). Transcriptome sequencing results revealed that alizarin inhibited DEV proliferation in DEF cells by modulating metabolic pathways such as those of tryptophan and arginine, thereby altering metabolites including indole and glutamate. (Conclusions）Alizarin effectively inhibits DEV proliferation in DEF cells and mitigates virus-induced cytopathic effects, thereby providing a theoretical foundation for the screening of targeted antiviral agents against DEV.