Role of AS-OCT in Assessment of Healing of Thin or Perforated Cornea Treated by Amniotic Membrane Graft and PRP Clot

Background: The purpose of the study is to evaluate the role of anterior segment optical coherence tomography in the assessment of the healing of corneal perforation after surgical treatment by amniotic membrane graft and platelet-rich plasma clot. Methods: Prospective, consecutive clinical series of cases. 13 eyes of 13 patients with small corneal perforation or thinning were included in the study. The patients were surgically treated with an amniotic membrane graft (AMG) and a platelet-rich plasma (PRP) clot, and the anterior chamber was reformed using air. Anterior segment optical coherence tomography (AS-OCT) was performed 1 week and 1 month after the surgery to confirm corneal healing and to assess the anterior chamber. Results: All cases demonstrated the formation of the anterior chamber and adequate intraocular pressure digitally during the follow-up period, and all cases showed complete healing of the corneal perforation by AS-OCT within the 4-week follow-up period. Conclusion AS-OCT is an essential tool to confirm healing of the corneal perforation surgically treated by amniotic membrane graft and platelet-rich plasma clot. Trial registration: The study was registered in ClinicalTrials.gov on May 20, 2024, with NCT06422897. The study was approved by the Ethics Committee, Faculty of Medicine, Minia University, El-Minya, Egypt (No. 1133/04/2024). This study adhered to the principles outlined in the Declaration of Helsinki.