Metabolomics-Based Analysis of the Correlation Between Metabolites and Thyroid Carcinoma

Backgrouds: The pathogenesis of thyroid carcinoma (TC) involves various factors, with the interplay between hormonal and environmental influences being particularly critical. The gut microbiome, a long-overlooked risk factor, may play a significant role in TC development. Studies indicate that gut dysbiosis, bacterial outer membrane components such as LPS, and metabolites like Short-Chain Fatty Acids (SCFAs)and Trimethylamine N-Oxide (TMAO) are pivotal in mediating or influencing both gastrointestinal and extra-gastrointestinal tumors. However, reports on the multi-omics analysis of the correlation between gut microbiota, metabolites, and thyroid carcinoma remain scarce. Methods: This study employs a case-control and cohort design, integrating 16S rDNA sequencing, and metabolomics for multi-omics joint analysis of fecal samples from thyroid cancer patients before and after surgery. The aim was to identify associations between the gut microbiome, functional genes, and metabolites, as well as potential disease biomarkers. Results: By combining existing clinical diagnostic indicators for thyroid carcinoma, this research aims to screen for novel biomarkers to aid in the diagnosis of thyroid carcinoma. Conclusions :This paper investigates the correlation between metabolites and thyroid carcinoma through metabolomics-based analysis. We speculate that the lipid metabolite cholesterol sulfate may affect thyroid cancer through key metabolic pathways such as steroid hormone synthesis and arachidonic acid metabolism.