Magneto-photoelectrochemical 2D heterojunction platform for biosensing detection

Photoelectrochemical (PEC) biosensors exhibit significant potential for biomolecule detection due to their high sensitivity and low background noise. However, their performance is severely constrained by the rapid recombination of photogenerated charge carriers. This study innovatively introduces a non-contact magnetic modulation strategy to suppress electron-hole recombination by manipulating carrier spin states, thereby significantly enhancing photoelectric conversion efficiency. Building on this mechanism, we developed a novel magnetically modulated PEC biosensing platform based on the MXenes/cobalt-doped titanium dioxide (Co-TiO ₂ ) heterostructure. This platform achieved ultrasensitive detection of protein kinase A (PKA) activity. Compared to an identical probe-modified biosensor without magnetic field application, the developed platform demonstrated a 68.75% enhancement in detection sensitivity and achieved an ultralow detection limit for PKA of 0.00016 U/mL. It also exhibited a wide linear range from 0.005 to 80 U/mL. This research not only provides a novel methodology for kinase activity analysis but also pioneers the innovative strategy of magnetic modulation for enhanced PEC sensing. It opens new avenues for developing high-performance biosensing platforms, holding significant promise for early disease diagnosis and drug screening applications.