The percentage of average relative abundances of Methylobacterium sp. Oral Taxon C7, Methylobacterium sp. Oral Taxon B84, and Methylobacterium hispanicumand association with periodontal health and type 2 diabetes in a cohort of Sri Lankan men diagnosed with oral fibroepithelial polyps

Objectives: The present study aimed to assess the percentage of average relative abundances of Methylobacterium sp. Oral Taxon C7, Methylobacterium sp. Oral Taxon B84, and Methylobacterium hispanicum and their association with periodontal health and type 2 diabetes in a cohort of Sri Lankan men diagnosed with oral fibroepithelial polyps. Materials and methods: This retrospective study was based a representative sub-sample of 25 clinically diagnosed oral FEP (the control group) selected from a main case-control study. Amplification of nucle­otides of Extracted DNA from frozen tissues was subjected to nucleotide amplification for the V1 to V3 region. The amplified nucleotides were subjected to sequencing with Illumina’s 2X 300–bp chemistry. The high-quality nonchimeric merged reads were classified to the species level with a prior­itized BLASTN-based algorithm. A structured, pre-tested interviewer administered questionnaire was used to collect socio-demographics, oral risk habits, oral hygiene indicators and medical history data. The data were entered and analyzed using the SPSS 21 Statistical Package. Descriptive statistics were presented as percentage distributions. Fisher’s exact test compared groups with cell counts less than 5. Results: The percentage of average relative abundance of Methylobacterium sp . Oral Taxon C7 was 0.06%, followed by Methylobacterium sp. Oral Taxon B84 was 0.03% and Methylobacterium hispanicum was 0.01%. There was no statistically significant association between the percentage of average relative abundance of each Methylobacterium species with periodontal disease status and T2DM. Conclusion: Though , there are statistically significance associations this study highlights the need of powered cohort studies to find out possible associations of this nosocomial pathogen with periodontal disease status and T2DM.