Comparative evaluation of sealing ability and cytotoxicity of Ceraseal versus AH plus sealers in obturated root canals using different obturation techniques (an in vitro study)

Objectives This study aimed to evaluate sealing ability of Ceraseal (BC) versus AH Plus (epoxy resin-based) sealers to root canals obturated with three different obturation techniques. and cytotoxicity of both sealers. Methods 42 freshly extracted single-rooted teeth were divided into six groups (each n n = 7). Group 1 (Ceraseal/CLC), Group 2 (Ceraseal/SC), Group 3 (Ceraseal/CW), Group 4 (AH Plus/ CLC), Group 5 (AH Plus/SC), and Group 6 (AH Plus/ CW). And instrumentation done. The sealer was fluorescently labeled by a fluorescent dye to allow analysis under confocal laser scanning microscopy (CLSM). Then specimens were obturated by different obturation techniques. Roots cross-sectioned perpendicular to their long axis, and then CLSM was employed to assess tubular penetration of different sealers. The cytotoxic effect of Ceraseal and AH Plus had been evaluated on the human gingival fibroblast cell line (HGF) using MTT assay test. One Way ANOVA test was used to compare different groups, followed by post hoc test for multiple section comparisons. Results Ceraseal/LC 5.27 ± 2.84 group followed by Ceraseal/SC 3.29 ± 1.07, AH+/CW 2.63 ± 0.62, and AH+/LC 3.10 ± 1.43, while the least were AH+/SC 2.40 ± 0.29 and Ceraseal/CW 1.86 ± 0.44, with no significant differences. The viability of cells using AH Plus sealer showed 20.3% and 24.52%, and 25.79%, and Ceraseal sealer was 71.39% and 76.15% at 24 h and 48 h; it then increased rather substantially to 90.41% at 72 h. revealed significant differences. Conclusion Obturation technique should be selected according to sealer type for a successful endodontic treatment. Ceraseal had higher ability of dentinal tubule penetration than AH Plus, reduced in apical part of root canal. Ceraseal expressed higher cell viability improved with time compared to AH Plus sealer.