Green Synthesis of Copper Nanoparticles with Adhatoda Vasica: Antibacterial and Antioxidant Study

The burgeoning field of green nanotechnology has spurred the interest of researchers towards environmentally responsible nanoparticle production. In this study, Aradusi leaf extract was utilized for the synthesis of stable copper nanoparticles, subsequently functionalized with Polyvinyl Pyrrolidone (PVP) polymer. A comprehensive characterization of these biosynthesized nanoparticles was conducted using UV–Visible spectrophotometry, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The UV–visible absorption spectra of bio-reduced copper nanoparticles were analyzed to assess their stability, while their antibacterial activity was evaluated against both gram-negative and gram-positive microbes. Additionally, their antioxidant potential was determined through DPPH free radical scavenging assays. Aradusi leaf extract demonstrated proficient reduction of copper ions into copper nanoparticles. Consequently, this methodology offers a rapid and environmentally benign route for the synthesis of stable copper nanoparticles exhibiting antibacterial and antioxidant activities within the size range of 10-100 nm, showcasing their potential applications in medical science.