Investigation of the Effects of Different Dietary Treatment Methods on Liver Apoptosis in Mice With Pentylenetetrazole Induced Epilepsy Model

Purpose: In this study, it was aimed to evaluate the effects of ketogenic diet, intermittent fasting and high-fat diet models on the liver in Balb-c mice in which epilepsy pentylenetetrazole (PTZ) kindling model was created. Materials and methods: 40 mice which is 8-week-old Balb/c were used in the study. To create a pentylenetetrazole kindling model, a total of 15 doses of 30mg/kg PTZl injection were administered every other day. Dietary practices were started simultaneously with the first PTZ injection. Mice; They were divided into 4 groups as standard rodent diet, high-fat diet, intermittent fasting and ketogenic diet. At the end of the three-month experimental period, all groups were sacrificed. Liver tissues were taken from mice and fixed in 4% formaldehyde solution and embedded in paraffin blocks. After deparaffinization procedures, 5 µm sections were taken on the microtome device. Immunohistochemistry with Cas3, Cas8, CHOP, GRP78 and TNF-alpha antibodies; Hematoxylin staining was performed to analyze the pathological status. Statistical analyzes were made in the Graphpad application by taking 20X images under the microscope. Graphs were created by comparing all groups with each other. Results: While the group with the highest expression of Cas3, Cas8 and TNF-a was the PTZ+KD group, the group with the highest expression of GRP78 was PTZ+HFD. When Cas3, Cas 8, TNF-a and GRP78 expressions were examined in the experimental groups, the lowest expression level was observed in the PTZ+IF group. Conclusion: We suggest that intermittent fasting diets can be used as an alternative, at least considering the benefits for the liver, but we emphasize the need for further studies.