Clinical Pathological Significance and Biological Function of APIP in Hepatocellular Carcinoma

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Abstract

Background & aims With a high incidence and fatality rate, hepatocellular carcinoma (HCC) is one of the most prevalent malignant tumors in the world. In addition to examining its clinical pathogenic importance in HCC, our goal is to study the expression of Apaf-1-interacting protein( APIP) in HCC and investigate its effects on the biological functions of HCC cells and the underlying mechanisms. Methods To determine the expression level and clinical significance of APIP in HCC, we employed internal tissue samples for Real-time quantitative polymerase chain reaction,(RT-qPCR) and immunohistochemistry(IHC) procedures. In order to verify the findings, we obtained external datasets. We investigated the upstream regulatory transcription factors of APIP and talked about the molecular mechanism of the biological role of APIP in HCC through experiments on cell proliferation, cell cycle, and cell apoptosis as well as scratch and transwell assays to assess the effects of APIP on the proliferation, apoptosis, migration, and invasion capabilities of HCC cells. Results We discovered that HCC tissues exhibited higher levels of APIP expression than nearby non-tumor tissues, and that APIP could differentiate HCC from healthy liver tissues. Additionally, the age, gender, and poor prognosis of individuals with HCC were linked to APIP expression. In the meantime, down regulating APIP may encourage HCC cells to undergo apoptosis and prevent their growth, migration, and invasion. Furthermore, the transcription factor Serum response factor(SRF) and APIP may have a regulatory connection. conclusion By influencing the proliferation, apoptosis, migration, and invasion of HCC cells, APIP plays a significant role in the formation and progression of HCC and may provide a novel therapeutic target.

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