Effects of minocycline on pulpal and periodontal healing of instantly replanted teeth

Purpose: The purpose of this study is to explore the effects and mechanisms of minocycline on pulpal and periodontal healing of instantly replanted teeth. Patients and methods: Human Dental Pulp Stem Cells (hDPSCs) and Human Periodontal Ligament Stem Cells (hPDLSCs) were isolated and identified. The cell counting kit-8 was used to explore the proliferation ability of hDPSCs in minocycline. qRT-PCR and Western Blot were performed to explore the mRNA and protein levels of VEGF, VEGFR1, VEGFR2, ALP, Runx2, EPHB4, β-catenin, and GSK-3β. The tubule formation experiments, ALP staining, and Alizarin Red staining were used to analyze the effect of minocycline on angiogenic and osteogenic induction. Thirty male Wistar rats were randomly divided into three groups. After the operation, they were observed for 4 weeks and euthanized. Micro-CT scanning, hematoxylin-eosin staining and immunohistochemical staining were used to evaluate the pulpal and periodontalhealing. Results: The mRNA and protein levels of angiogenic-related factors, Wnt/β-catenin pathways-related factors, tubule formation experiments, ALP staining, Alizarin Red staining, BMD, hematoxylin-eosin staining and immunohistochemical staining were significantly increased in 10mg/L minocycline groups. Conclusions: We concluded that minocycline could promote pulpaland periodontalhealing of instantly replanted teeth in vivo and in vitro.