Phytochemical analysis of Citrus japonica enrich extract and investigation of Anti-inflammatory activity in Rat Model

Tangeretin (TAN), a naturally occurring polymethoxylated flavone derived from Citrus japonica peel, exhibited wide biomedical applications. Tangeretin is a hydrophobic compound, it has low solubility and availability, which limits its therapeutic potential. The in vivo anti-inflammatory effect of Tangeretin rich extract (TRE) & Tangeretin loaded nanoparticles (TNPs) (200µg/kg, 400µg/kg, 800µg/kg) were assessed against diclofenac and ibuprofen (10mg/kg) in adult male and female Wister rats using carrageenan and formaldehyde induced inflammation model. The effectiveness of both Tangeretin rich extract and Tangeretin loaded nanoparticles on formaldehyde evoked hallmarks were assessed through biochemical, hematological, radio-graphical and histopathological examination. The ELSA was carried out to assess the expression of interleukin-6 and TNF-α. Level of antioxidant enzymes in liver tissue homogenate were also assessed, the concentration of CRP and RA factor also examined. Furthermore, qPCR was used to access the regulatory effect on inflammatory biomarkers. Tangeretin rich extract and Tangeretin loaded nanoparticles significantly elevate (p <0.001) the level of antioxidant enzyme in contrast to pathological inflammation group. Tangeretin rich extract and Tangeretin-loaded nanoparticles significantly decreased paw swelling and paw diameter (p <.001) in formaldehyde and carrageenan induced inflammation model. Tangeretin rich extract and Tangeretin loaded nanoparticles significantly suppressed the Messenger Ribonucleic Acid expression of pro-inflammatory biomarker also reduced the concentration of antioxidant enzymes in liver tissue homogenate. Therefore, work proved Tangeretin rich extract and Tangeretin loaded nanoparticles possess great potential to prevent and treat inflammatory ailments.