Callus production and Micropropagation and Phytochemical Investigation of Corallocarpus epigaeus (Arn.) Cl. – A Potential Antidiabetic Medicinal Plant

The present study deals with the in vitro callus induction, micropropagation and phytochemical analysis of Corallocarpus epigaeus (Arn.) Cl. The tuber, node and leaf were used as explants and cultured on MS medium with NAA, 2,4-D, BAP and KN alone and different combinations for callus induction and micropropagation. The best callus formation of tuber explants was obtained on BAP: NAA after 20 days of inoculation while the nodal explants show best callus formation on BA: NAA after 10 days. Leaf explants not show callus induction in any combination. The nodal explants were selected for shoot multiplication and inoculated on MS media supplemented with the combination (BAP and IBA) of PGR shows the positive results of shoot multiplication and maximum shoot obtained after 15 days of inoculation. NAA of PGR shows the positive results of root production and maximum root and length obtained after 25 days of inoculation. After 25-day callus of tuber explants was collected and subjected to phytochemical investigation which indicates the presence of alkaloids, flavonoids, Phenols, steroids and saponins. The yield of alkaloids, flavonoids and Phenols are 4.71%, 0.23% and 0.013% respectively. Further study required for the large-scale production of secondary metabolites through callus production and also large-scale production of propagules required the standardization.