Association of CYP1A1 Rs1048943 Polymorphism with Male Infertility: A Study in East Azerbaijan, Iran

Introduction: Infertility is a disease of the reproductive system that is defined as non-pregnancy after 46 months of regular sexual intercourse without the use of contraceptive methods. Male infertility occurs in approximately 45% of couples. Various environmental and genetic factors play a role in male infertility. Among the genes involved in male infertility is the CYP1A1 gene, whose various polymorphisms are involved in infertility, including asthenospermia, which is one of the most important polymorphisms related to this polymorphic gene RS1048943. In this study, these polymorphisms in infertile men in northwestern Iran have been investigated. Methods For this purpose, sperm samples were prepared from Tabriz University Jihad Center and then DNA extraction was performed. Among the extracted DNAs, each sample of the desired quality was stored for other steps. Tetra ARMS PCR was used to study these polymorphisms. To perform PCR using primers designed for this gene, the desired region was amplified and after running on the gel, the genotypes were determined based on the obtained bands and statistical analyzes were performed using Excell software. P < 0.05 was significant. Results According to statistical studies, the percent of TT, AA and AT genotypes in the patient group was 52%, 4% and 44%, respectively, and the percent of genotypes in the control group was 32%, 52% and 16%, respectively. Discussion According to the results, genotypic frequency in the patient and control groups was insignificant and also the amount of allelic frequency in the patient and control groups was also insignificant.