Biochemical Assessment of Hepatic and Renal Functions Following Administration of Doses of Monosodium Glutamate and Shilajit in Albino Mice

Monooxyglutamate (MSG) is one of the most prominent additives used in the food industry to enhance food products. Although research has noted that chronic consumption of MSG may lead to oxidative imbalance in young body tissues, particularly in the liver and kidneys, by stimulating the production of free radicals or reactive oxygen species (ROS), the current study aimed to investigate the biochemical effects on hepatitis caused by different doses of MSG and an aqueous extract of shilajit in albino mice. fifty adult rats were randomly distributed into five equal groups (10 rats each). The first group (G1) received distilled water, the second group (G2) received 2 g/kg body weight of monosodium glutamate (MSG), (G3) received 2 g/kg body weight of MSG and 100 mg/kg of Shilajit the third, group (G4) received 4 g/kg body weight of MSG, the fourth group, and the fifth group (G5) received 4 g/kg body weight of MSG and 200 mg/kg of Shilajit. The oral treatments continued daily for 14 days. On the following day (14), the animals were euthanized and samples were taken for biochemical tests, which included ALT, AST, GGT and ALP enzymes, in addition to renal indices such as urea and creatinine.The results showed a significant increase below the P ≤ 0.05 level in liver enzymes, namely ALP, AST, and ALT, in the (G2) received 2 g/kg body weight of (MSG) and group (G4) received 4 g/kg body weight of MSG, while we noticed a significant increase in the GGT enzyme in thegroup (G3) received 2 g/kg body weight of MSG and 100 mg/kg of Shilajit and group (G5) received 4 g/kg body weight of MSG and 200 mg/kg of Shilajit, The results related to kidney functions also showed a significant increase in urea in the the (G2) received 2 g/kg body weight of (MSG) and group (G4) received 4 g/kg body weight of MSG, and a decrease in them in in thegroup (G3) received 2 g/kg body weight of MSG and 100 mg/kg of Shilajit and group (G5) received 4 g/kg body weight of MSG and 200 mg/kg of Shilajit, The results indicate the toxicity of monosodium glutamate on the liver and kidneys and the effectiveness of the aqueous extract of shilajit to reduce toxicity.