Quercetin protects against sepsis-induced myocardial injury through RIPK1/RIPK3/MLKL pathway in juvenile mice
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Background: To study the effect of Quercetin on sepsis-induced myocardial injury through regulating RIPK1/RIPK3/MLKL pathway in juvenile mice. Methods: The mouse model of septic cardiomyopathy (SCM) was established by intraperitoneal injection of LPS. Besides, Quercetin was injected, or RIPK1 inhibitor Necrostatin-1 (Nec-1) co-injected before modeling. Echocardiography was employed to assess the cardiac function of mice. Myocardial injury markers in mice were quantified using an automated biochemical analyzer. The concentrations of inflammatory factors in both serum and myocardial tissue of mice were measured via ELISA. TEM was utilized to examine the micro-structure of myocardial cells. The expression of RIPK1/RIPK3/MLKL signaling pathway proteins in the myocardial tissue of mice was detected by WB. Results: Quercetin treatmentimprove the cardiac function, reduce the levels of myocardial injury markers and inflammatory factors in SCM mice. Moreover, The expression of RIPK1/RIPK3/MLKL signaling pathway proteins were decreased after Quercetin treatment. Interesting, the combination of Nec-1 and Quercetin could reduce the levels of myocardial injury markers and inflammatory factors in SCM mice. Conclusion: Quercetin can alleviate myocardial injury in juvenileSCM mice by inhibiting the RIPK1/RIPK3/MLKL signaling pathway.
