Antimicrobial activity, ergosterol content and phytochemical screening of Rorippa islandica (Oeder ex Murr.) and Carrichtera annua (L.)

The purpose of this study is to identify non-polluting substitute medicinal plants. This study interested by two medicinal plants ( Rorippa islandica and Carrichtera annua ) belonging to family Brassicaceae, to identify their bioactive constituents in their ethanol crude extracts using GC-MS and HPLC techniques, and evaluated in vitro their antifungal activity against five pathogenic fungi. GC-MS detected the existence of 50 and 55 compounds in Rorippa islandica and Carrichtera annua plant, respectively. The primary compound was 13-Docosenamide (20.54%) at Rorippa islandica and 2-Hydroxy-1-(Hydroxymethyl) Ethyl Stearate (9.74%) at Carrichtera annua , while HPLC identified 19 and 18 phenolic compounds in Rorippa islandica and Carrichtera annua , respectively. Gallic acid was the main compound in the two plants with concentration (3417.72 µg/g and 3733.98 µg/g), respectively. On the other hand, both plant extracts revealed potential antifungal activity, where the most promising effect of Rorippa islandica at concentration (10 mg/ml) was for ethanol 70% successive extract against Colletotrichum gloeosporioides with antimicrobial activity, MIC, and MFC (29±0.3 mm,7.8 µg/ml and 15.62 µg/ml), respectively. The most promising effect of Carrichtera annua L. at concentration (10 mg/ml) was for total extract against Curvularia lunata with antimicrobial activity, MIC and MFC (35±0.1 mm, 1.97 µg/ml and 3.9 µg/ml), respectively. A significant reduction in the ergosterol content of total and two ethanol (96% & 70%) successive fractions of two plants, the highest ergosterol reduction was in Curvularia lunata and Colletotrichum gloeosporioides with Carrichtera annua extract (49.93%, 47.7%), respectively. Penicillium glabrum and Colletotrichum gloeosporioides with Rorippa islandica extract (47.2%, 42.58%), respectively. The changes in cell morphology induced by total and ethanol (96% &70%) extracts of two plants were examined using AFM.