Evaluation of the effects of the combination of xenogeneic bone grafts with autologous platelet concentrates prepared using different centrifugation protocols on the treatment of critical-size bone defects in rat calvaria: microtomographic and histomorphometric analyses at a late healing stage

  1. Wanderson Thalles de Souza Braga
  2. Ulli da Costa Cunha Martins
  3. Débora de Souza Ferreira Sávio
  4. Lucia Moitrel Pequeno da Silva
  5. Maria da Conceição Pereira Saraiva
  6. Flávia Furlaneto
  7. Carlos Fernando Mourão
  8. Richard Miron
  9. Roberta Okamoto
  10. Michel Messora
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Abstract

Background This study assessed the impact of xenogeneic bone grafts (XEN), either utilized alone or combined with autologous platelet concentrates (APCs) prepared using three different centrifugation protocols on healing of critical-size defects (CSDs) in rat calvaria. Methods Forty rats were assigned to five groups (n = 8): Control, XEN, XEN + L-PRF, XEN + A-PRF, and XEN + Bio-PRF. Blood concentrates were prepared by centrifuging blood collected via cardiac puncture: L-PRF (~ 700g for 12 minutes), A-PRF (~ 208g for 14 minutes), and Bio-PRF (~ 700g for 8 minutes). Defects (5 mm diameter) in the right parietal bone of each rat were filled with blood clot, XEN or XEN combined with L-PRF, A-PRF, or Bio-PRF. Animals were euthanized 60 days post-surgery and the calvariae were analyzed using histomorphometry and micro-CT. Results Groups XEN-A-PRF, XEN-L-PRF and XEN-BIO-PRF presented higher bone volume (BV) and lower bone porosity (Po) than the control group (p < 0.05). The XEN group did not show different values of VO and Po compared to the control group. Only the XEN + A-PRF and XEN + Bio-PRF groups showed a higher percentage of mature collagen fibers (red color) compared to the XEN and control groups (p < 0.05). The XEN group showed a higher amount of immature collagen fibers (green color) compared to the XEN + A-PRF, XEN + L-PRF, and XEN + Bio-PRF groups (p < 0.05). Conclusions It can be concluded that CSDs treated with XEN combined with A-PRF or Bio-PRF, in late-stage healing assessments, presented higher newly formed bone tissue with greater collagen maturation than those treated with XEN alone; ii) the centrifugation protocol used for preparing APCs seems to be a decisive factor for the quality of the collagen matrix in newly formed bone tissue.

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  1. Version published to 10.21203/rs.3.rs-6631987/v1 on Research Square